Plasmid pBR322 has PstI restriction enzyme site within gene…

Biology Biotechnology Principles and Processes Previous Year Questions Single Correct MCQ

Published on: August 14, 2026

Plasmid pBR322 has PstI restriction enzyme site within gene amp R that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain

it will not be able to confer ampicillin resistance to the host cell.

the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.

it will lead to lysis of host cell.

it will be able to produce a novel protein with dual ability

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The correct answer is:

Insertion of foreign DNA at the PstI site within the amp R gene of pBR322 disrupts (inactivates) the ampicillin resistance gene, so the recombinant plasmid loses ampicillin resistance.

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